Product description The MethylFlash ™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of buffers and reagents optimized to colorimetrically quantify the global methylation status of DNA by specifically measuring 5-methylcytosine (5-mC) levels in a simplified format. , “one-step” reaction similar to ELISA. This makes it possible to directly measure global DNA … Read more
Product description The FitAmp ™ Blood and Cultured Cell DNA Extraction Kit is a comprehensive set of essential components designed for the rapid isolation of pure genomic DNA. The researcher can quickly and efficiently isolate DNA from blood leukocytes and cultured mammalian cells. The entire procedure can be completed in 20 minutes. Fast: the entire … Read more
The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as … Read more
The antibody is provided as 100 uL of affinity-purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide. Reacts with mouse, rat, and rabbit species. The images above labeled “ImmunoStar Rabbit 5-HT 2A receptor antibody” are the results of staining … Read more
The histochemical antibody for the 5-HT1A receptor is generated in a rabbit against a synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity-purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods. The … Read more
The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar … Read more
Pre-adsorption of Serotonin antisera, diluted according to the antibody specification sheet, with 20 µg/ml Serotonin/BSA conjugate following the instructions below provides complete blockage of Serotonin immunolabeling. The conjugate is provided as 50 µg of lyophilized Serotonin creatinine sulfate coupled to BSA with paraformaldehyde. Also, this antiserum contains 0.09% sodium azide. Please read the instructions carefully … Read more