A nucleic acid-based orthopoxvirus vaccine targeting the vaccinia virus L1, A27, B5 and A33 proteins protects rabbits against lethal rabbitpox virus aerosol challenge

In the age of COVID, nucleic acid vaccines have garnered much attention, at least in part, because of the simplicity of construction, production, and flexibility to adjust and adapt to an evolving outbreak. Orthopoxviruses remain a threat on multiple fronts, especially as emerging zoonosis. In response, we developed a DNA vaccine, termed 4pox, that protected nonhuman primates against monkeypox virus (MPXV) induced severe disease. Here, we examined the protective efficacy of the 4pox DNA vaccine delivered by intramuscular (i.m.) electroporation (EP) in rabbits challenged with aerosolized rabbitpox virus (RPXV), a model that recapitulates the respiratory route of exposure and low dose associated with natural smallpox exposure in humans.
We found that 4pox vaccinated rabbits developed immunogen-specific antibodies, including neutralizing antibodies and did not develop any clinical disease, indicating protection against aerosolized RPXV. In contrast, unvaccinated animals developed significant signs of disease, including lesions, and were euthanized. These findings demonstrate that an unformulated, non-adjuvanted DNA vaccine delivered (i.m.) can protect against an aerosol exposure.
Importance The eradication of smallpox and subsequent cessation of vaccination has left a majority of the population susceptible to variola virus or other emerging poxvirus. This is exemplified by human monkeypox, as evidenced by the increase in reported endemic and imported cases over the past decades.
Therefore, a malleable vaccine technology that can be mass-produced, and doesn’t require complex conditions for distribution and storage is sought. Herein, we show that a DNA vaccine, in the absence of a specialized formulation or adjuvant, can protect against a lethal aerosol insult of rabbitpox virus.

Asymptomatic Orthopoxvirus Circulation in Humans in the Wake of a Monkeypox Outbreak among Chimpanzees in Cameroon

Monkeypox virus is a zoonotic Orthopoxvirus (OPXV) that causes smallpox-like illness in humans. In Cameroon, human monkeypox cases were confirmed in 2018, and outbreaks in captive chimpanzees occurred in 2014 and 2016. We investigated the OPXV serological status among staff at a primate sanctuary (where the 2016 chimpanzee outbreak occurred) and residents from nearby villages, and describe contact with possible monkeypox reservoirs. We focused specifically on Gambian rats (Cricetomys spp.) because it is a recognized possible reservoir and because contact with this species was common enough to render sufficient statistical power.
We collected one 5-mL whole blood specimen from each participant to perform a generic anti-OPXV ELISA test for IgG and IgM antibodies and administered a questionnaire about prior symptoms of monkeypox-like illness and contact with possible reservoirs. Our results showed evidence of OPXV exposures (IgG positive, 6.3%; IgM positive, 1.6%) among some of those too young to have received smallpox vaccination (born after 1980, n = 63). No participants reported prior symptoms consistent with monkeypox.
After adjusting for the education level, participants who frequently visited the forest were more likely to have recently eaten Gambian rats (OR: 3.36, 95% CI: 1.91-5.92, P < 0.001) and primate sanctuary staff were less likely to have touched or sold Gambian rats (OR: 0.23, 95% CI: 0.19-0.28, P < 0.001). The asymptomatic or undetected circulation of OPXVs in humans in Cameroon is likely, and contact with monkeypox reservoirs is common, raising the need for continued surveillance for human and animal diseases.

Co-administration of tecovirimat and ACAM2000 in non-human primates: Effect of tecovirimat treatment on ACAM2000 immunogenicity and efficacy versus lethal monkeypox virus challenge.

Naturally occurring smallpox has been eradicated but research stocks of variola virus (VARV), the causative agent of smallpox, still exist in secure laboratories. Clandestine stores of the virus or resurrection of VARV via synthetic biology are possible and have led to concerns that VARV could be used as a biological weapon. The US government has prepared for such an event by stockpiling smallpox vaccines and TPOXX, SIGA Technologies’ smallpox antiviral drug. While vaccination is effective as a pre-exposure prophylaxis, protection is limited when administered following exposure. Safety concerns preclude general use of the vaccine unless there is a smallpox outbreak.
TPOXX is approved by the FDA for use after confirmed diagnosis of smallpox disease. Tecovirimat, the active pharmaceutical ingredient in TPOXX, targets a highly conserved orthopoxviral protein, inhibiting long-range dissemination of virus. Although indications for use of the vaccine and TPOXX do not overlap, concomitant use is possible, especially if the TPOXX indication is expanded to include post-exposure prophylaxis.
It is therefore important to understand how vaccine and TPOXX may interact. In studies presented here, monkeys were vaccinated with the ACAM2000TM live attenuated smallpox vaccine and concomitantly treated with tecovirimat or placebo. Immune responses to the vaccine and protective efficacy versus a lethal monkeypox virus (MPXV) challenge were evaluated. In two studies, primary and anamnestic humoral immune responses were similar regardless of tecovirimat treatment while the third study showed reduction in vaccine elicited humoral immunity.
Following lethal MPXV challenge, all (12 of 12) vaccinated/placebo treated animals survived, and 12 of 13 vaccinated/tecovirimat treated animals survived. Clinical signs of disease were elevated in tecovirimat treated animals compared to placebo treated animals. This suggests that TPOXX may affect the immunogenicity of ACAM2000 if administered concomitantly. These studies may inform on how vaccine and TPOXX are used during a smallpox outbreak.

Use of live Variola virus to determine whether CAST/EiJ mice are a suitable surrogate animal model for human smallpox

Numerous animal models of systemic orthopoxvirus disease have been developed to evaluate therapeutics against variola virus (VARV), the causative agent of smallpox. These animal models do not resemble the disease presentation in human smallpox and most used surrogate Orthopoxviruses. A rodent model using VARV has a multitude of advantages, and previous investigations identified the CAST/EiJ mouse as highly susceptible to monkeypox virus infection, making it of interest to determine if these rodents are also susceptible to VARV infection.

Recombivirus Monkey Anti-monkeypox IgG ELISA kit, 96 tests, Quantitative

AE-311150-1 Alpha Diagnostics 1 kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative

RV-403210-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) NS1 protein IgG ELISA kit, 96 tests, Quantitative

RV-403310-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Envelop protein IgG ELISA kit, 96 tests, Quantitative

RV-403110-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Capsid protein IgG ELISA kit, 96 tests, Quantitative

RV-403410-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Envelop domain III IgG ELISA kit, 96 tests, Quantitative

RV-403160-1 Alpha Diagnostics 1 Kit

RecombiVirus Monkey EDIM/rotavirus VP6 antibody IgG ELISA Kit, 96 tests, Quantitative

AE-300440-1 Alpha Diagnostics 1 kit

Recombivirus Monkey Corona virus (Bcov/BCV) nucleocapsid protein IgG ELISA kit, 96 tests

RV-500530-1 Alpha Diagnostics 1 kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Env+NS1+Prm+Capsid antibody Combo IgG ELISA kit, 96 tests, Quantitative

RV-403460-C Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) PrM protein IgM ELISA kit, 96 tests, Quantitative

RV-403215-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) NS1 protein IgM ELISA kit, 96 tests, Quantitative

RV-403315-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Envelop protein IgM ELISA kit, 96 tests, Quantitative

RV-403115-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Capsid protein IgM ELISA kit, 96 tests, Quantitative

RV-403415-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Envelop domain III IgM ELISA kit, 96 tests, Quantitative

RV-403165-1 Alpha Diagnostics 1 Kit

Recombivirus? Monkey Anti-Zika Virus (ZIKV) Env+NS1+Prm+Capsid antibody Combo IgM ELISA kit, 96 tests, Quantitative

RV-403465-C Alpha Diagnostics 1 Kit

Recombinant Monkeypox Virus antigen

E41H094 EnoGene 100ug

Recombinant Monkeypox Virus antigen

E41H095 EnoGene 100ug

Monkeypox Virus -MPXV- IgG ELISA Kit

E-EL-E620-24Tests Elabscience Biotech 24 Tests

Monkeypox Virus -MPXV- IgG ELISA Kit

E-EL-E620-48Tests Elabscience Biotech 48 Tests

Monkeypox Virus -MPXV- IgG ELISA Kit

E-EL-E620-96Tests Elabscience Biotech 96 Tests

Monkeypox Virus -MPXV- IgG ELISA Kit

E-EL-E620-96Tests10 Elabscience Biotech 96 Tests *10

Monkeypox Virus -MPXV- IgG ELISA Kit

E-EL-E620-96Tests5 Elabscience Biotech 96 Tests *5

Human Monkeypox Virus IgG (MPXV IgG) ELISA Kit

abx092187-100l Abbexa 100 µl

Human Monkeypox Virus IgG (MPXV IgG) ELISA Kit

abx092187-1ml Abbexa 1 ml

Human Monkeypox Virus IgG (MPXV IgG) ELISA Kit

abx092187-200l Abbexa 200 µl

Human Monkeypox Virus IgG (MPXV IgG) ELISA Kit

abx392298-100g Abbexa 100 µg

Recombinant Monkeypox virus A29 Protein

RP01543 Abclonal 100μg

Recombinant Monkeypox virus L1R Protein

RP01544 Abclonal 500μg

Recombinant Monkeypox virus A29 Protein

RP01546 Abclonal 50μg

Recombinant Monkeypox virus D6L Protein

RP01606 Abclonal 50μg

Recombinant Monkeypox virus A30L Protein

RP01593 Abclonal 20μg

Recombinant Monkeypox virus B16R Protein

RP01594 Abclonal 50μg

Human Monkeypox Virus M1R IgG (MPXV M1R IgG) ELISA Kit

abx395182-100g Abbexa 100 µg

Human Monkeypox Virus H3L IgG (MPXV H3L IgG) ELISA Kit

abx395183-25g Abbexa 25 µg

Human Monkeypox Virus E8L IgG (MPXV E8L IgG) ELISA Kit

abx395184-50g Abbexa 50 µg

Recombinant Monkeypox Virus M1R Protein [His]

DAG-WT758 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus B6R Protein [His]

DAG-WT761 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus B6R Protein [His]

DAG-WT773 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus H3L Protein [His]

DAG-WT776 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus M1R Protein [His]

DAG-WT779 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus A29L Protein [His]

DAG-WT759 Creative Diagnostics 1 mg

Recombinant Monkeypox Virus A35R Protein [His]

DAG-WT760 Creative Diagnostics 1 mg

Human Monkeypox Virus A29L IgG (MPXV A29L IgG) ELISA Kit

abx395181-50g Abbexa 50 µg

Human Monkeypox Virus A35R IgG (MPXV A35R IgG) ELISA Kit

abx395185-100g Abbexa 100 µg

Recombivirus Rabbit Anti-rabbitpox (RPXV090) IgG ELISA kit, 96 tests

AE-311120-1 Alpha Diagnostics 1 kit

Recombivirus Horse Anti-horsepox IgG ELISA kit, 96 tests, Quantitative

AE-311140-1 Alpha Diagnostics 1 kit

Human Monkeypox Virus IgG/IgM (MPXV IgG/IgM) Rapid Test Kit

abx092183-100l Abbexa 100 µl

Human Monkeypox Virus IgG/IgM (MPXV IgG/IgM) Rapid Test Kit

abx092183-1ml Abbexa 1 ml

Human Monkeypox Virus IgG/IgM (MPXV IgG/IgM) Rapid Test Kit

abx092183-200l Abbexa 200 µl

Recombinant Monkeypox virus A33R protein (His tag)

PDEV100007-100ug Elabscience Biotech 100ug

Recombinant Monkeypox virus A33R protein (His tag)

PDEV100007-20ug Elabscience Biotech 20ug

Monkeypox B5R

mpv-002 ProSpec Tany 100µg

Monkeypox A33R

mpv-001 ProSpec Tany 100µg

Monkeypox virus

Oneq-VH747-OneqVH747100D Bioingentech Oneq-VH747-100D

Monkeypox virus

Oneq-VH747-OneqVH747150D Bioingentech Oneq-VH747-150D

Monkeypox virus

Oneq-VH747-OneqVH74750D Bioingentech Oneq-VH747-50D

Recombinant Monkeypox virus A26L protein (His,SUMO tag)

PDEV100008-100ug Elabscience Biotech 100ug

Recombinant Monkeypox virus A26L protein (His,SUMO tag)

PDEV100008-20ug Elabscience Biotech 20ug

Recombinant Monkeypox virus MPXV(A29L) protein (His tag)

PDEV100009-100ug Elabscience Biotech 100ug

Recombinant Monkeypox virus MPXV(A29L) protein (His tag)

PDEV100009-20ug Elabscience Biotech 20ug

RecombiVirus Mouse Parvovirus (MPV) IgG ELISA Kit, 96 tests

AE-300500-1 Alpha Diagnostics 1 kit

Recombinant Monkeypox Virus Chimeric Protein (B21R&A29L)

DAG-WT764 Creative Diagnostics 1 mg

RecombiVirus Rat Minute Virus (MVM) IgG ELISA Kit, 96 tests

AE-300910-1 Alpha Diagnostics 1 kit

RecombiVirus Rat Pneumonia Virus (PVM) IgG ELISA Kit, 96 tests

AE-310410-1 Alpha Diagnostics 1 kit

RecombiVirus Sheep EDIM/rotavirus VP6 antibody IgG ELISA Kit, 96 tests

AE-300460-1 Alpha Diagnostics 1 kit

RecombiVirus Mouse Norovirus 1 (MNV-1) IgG ELISA Kit, 96 tests

AE-300300-1 Alpha Diagnostics 1 kit

Monkeypox Virus B2R Recombinant Protein N-His Tag Lyophilized

IMPXVB2RRNHISLY100UG Innovative research each

Monkeypox Virus B2R Recombinant Protein N-His Tag Lyophilized

IMPXVB2RRNHISLY1MG Innovative research each

RecombiVirus Mouse Anti-Seneca Valley Virus (SeV) IgG ELISA kit, Quantitative, 96 tests

RV-404220-1 Alpha Diagnostics 1 kit

Monkeypox Virus A35R Recombinant Protein C-His Tag Lyophilized

IMPXVA35RRCHISLY100UG Innovative research each

Monkeypox Virus A35R Recombinant Protein C-His Tag Lyophilized

IMPXVA35RRCHISLY1MG Innovative research each

Monkeypox Virus C18L Recombinant Protein N-His Tag Lyophilized

IMPXVC18LRNHISLY100UG Innovative research each

Monkeypox Virus C18L Recombinant Protein N-His Tag Lyophilized

IMPXVC18LRNHISLY1MG Innovative research each

Monkeypox Virus C19L Recombinant Protein N-His Tag Lyophilized

IMPXVC19LRNHISLY100UG Innovative research each

Monkeypox Virus C19L Recombinant Protein N-His Tag Lyophilized

IMPXVC19LRNHISLY1MG Innovative research each

RecombiVirus Rat sialodacryoadenitis Virus (SDAV) IgG ELISA Kit, 96 tests

AE-310310-1 Alpha Diagnostics 1 kit

Recombivirus Rat Hantavirus Nucleoprotein (HVNP) IgG ELISA Kit, 96 tests

AE-310610-1 Alpha Diagnostics 1 kit

RecombiVirus Rat Toolan's H-1 Virus (H-1/THV) IgG ELISA Kit, 96 tests

AE-310120-1 Alpha Diagnostics 1 kit

RecombiVirus Rat Kilham Virus (KRV) IgG ELISA Kit, 96 tests, Quantitative

AE-310110-1 Alpha Diagnostics 1 Kit

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-MP848659MHV Cusabio 20μg

Recombinant Monkeypox virus Envelope protein A28 homolog (A30L), partial

CSB-EP848659MHV Cusabio 10523 mg

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-EP848659MHV-B Cusabio 20μg

Recombinant Monkeypox virus Envelope protein A28 homolog (A30L), partial

CSB-EP848659MHVb1 Cusabio 11272 mg

Recombinant Monkeypox virus Envelope protein A28 homolog(A30L),partial

CSB-BP848659MHV Cusabio 20μg

Recombivirus? Human Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative

RV-403200-1 Alpha Diagnostics 1 Kit

Recombivirus? Mouse Anti-Zika Virus (ZIKV) PrM protein IgG ELISA kit, 96 tests, Quantitative

RV-403220-1 Alpha Diagnostics 1 Kit

Recombivirus? Rabbit Anti-Zika Virus (ZIKV) prM protein IgG ELISA kit, 96 tests, Quantitative

RV-403230-1 Alpha Diagnostics 1 Kit

Recombivirus? Human Anti-Zika Virus (ZIKV) NS1 protein IgG ELISA kit, 96 tests, Quantitative

RV-403300-1 Alpha Diagnostics 1 Kit

Recombivirus? Mouse Anti-Zika Virus (ZIKV) NS1 protein IgG ELISA kit, 96 tests, Quantitative

RV-403320-1 Alpha Diagnostics 1 Kit

Recombivirus? Rabbit Anti-Zika Virus (ZIKV) NS1 protein IgG ELISA kit, 96 tests, Quantitative

RV-403330-1 Alpha Diagnostics 1 Kit

Recombivirus Mouse Hantavirus Nucleoprotein (HVNP) IgG ELISA Kit, 96 tests

AE-310600-1 Alpha Diagnostics 1 kit

RecombiVirus Rat Hepatitis virus (MHV/Coronavirs) IgG ELISA Kit, 96 tests

AE-300710-1 Alpha Diagnostics 1 kit

RecombiVirus Porcine rotavirus VP6 antibody IgG ELISA Kit, 96 tests, Quantitative

AE-300470-1 Alpha Diagnostics 1 kit

RecombiVirus Mouse Minute Virus (MVM) IgG ELISA Kit, 96 tests, Quantitative

AE-300900-1 Alpha Diagnostics 1 Kit

Recombivirus Camelpox virus H3L/p35 IgG ELISA kit, 96 tests, Quantitative

AE-311170-1 Alpha Diagnostics 1 kit

Recombivirus? Camel Anti-Peste des petits ruminants NP (PPR-NP) IgG positive control serum

RV-400820-06P Alpha Diagnostics 1 ml

Recombivirus? Human Anti-Zika Virus (ZIKV) Capsid protein IgG ELISA kit, 96 tests, Quantitative

RV-403400-1 Alpha Diagnostics 1 Kit

Recombivirus? Mouse Anti-Zika Virus (ZIKV) Capsid protein IgG ELISA kit, 96 tests, Quantitative

RV-403420-1 Alpha Diagnostics 1 Kit

Monkeypox Virus, 20 tests/kit

U202028-20 USTAR Biotechnologies (Hangzhou) test

RecombiVirus Mouse Hepatitis virus (MHV/Coronavirs) IgG ELISA Kit, 96 tests

AE-300700-1 Alpha Diagnostics 1 kit

Recombivirus Rat Reovirus sigma-3 (Reo-3/REOV) IgG ELISA Kit, 96 tests

AE-310710-1 Alpha Diagnostics 1 kit

MonkeyPox Virus (MPV) ELISA Kit

SL0026Mk Sunlong -

MonkeyPox Virus (MPV) ELISA Kit

YX-SL0036Mk Sunlong -

Recombivirus Mouse Anti-Cytomegalovirus (MCMV/MuHV-1) gB IgG ELISA Kit, 96 tests, Quantitative

AE-301000-1 Alpha Diagnostics 1 Kit

Recombivirus? Human Anti-Zika Virus (ZIKV) Envelop protein IgG ELISA kit, 96 tests, Quantitative

RV-403100-1 Alpha Diagnostics 1 Kit

Recombivirus? Mouse Anti-Zika Virus (ZIKV) Envelop protein IgG ELISA kit, 96 tests, Quantitative

RV-403120-1 Alpha Diagnostics 1 Kit

Recombivirus? Rabbit Anti-Zika Virus (ZIKV) Envelop protein IgG ELISA kit, 96 tests, Quantitative

RV-403130-1 Alpha Diagnostics 1 Kit

Recombivirus? Rabbit Anti-Zika Virus (ZIKV) Capsid protein IgG ELISA kit, 96 tests, Quantitative

RV-403430-1 Alpha Diagnostics 1 Kit

RecombiVirus Rat Polyomavirus (KV, Pneumotropic virus) IgG ELISA Kit, 96 tests

AE-300110-1 Alpha Diagnostics 1 kit

RecombiVirus Mouse Pneumonia Virus (PVM) IgG ELISA Kit, 96 tests, Quantitative

AE-310400-1 Alpha Diagnostics 1 Kit

RecombiVirus Human EDIM/rotavirus VP6 antibody IgG ELISA Kit, 96 tests, Quantitative

AE-300430-1 Alpha Diagnostics 1 kit

Recombivirus Mouse Reovirus sigma-3 (Reo-3/REOV) IgG ELISA Kit, 96 tests

AE-310700-1 Alpha Diagnostics 1 kit

Monkeypox Virus H3L (MPXV H3L) Antibody

abx376562-96tests Abbexa 96 tests

Monkeypox Virus M1R (MPXV M1R) Antibody

abx376563-96tests Abbexa 96 tests

Monkeypox Virus M1R (MPXV M1R) Antibody

abx376564-96tests Abbexa 96 tests

Mouse Anti Monkeypox Virus E8L Monoclonal Clone SAA0285

IMSAMPXVE8LSAA0285C100UG Innovative research each

Mouse Anti Monkeypox Virus E8L Monoclonal Clone SAA0285

IMSAMPXVE8LSAA0285C1MG Innovative research each

Mouse Anti Monkeypox Virus H3L Monoclonal Clone SAA0282

IMSAMPXVH3LSAA0282C100UG Innovative research each

Mouse Anti Monkeypox Virus H3L Monoclonal Clone SAA0282

IMSAMPXVH3LSAA0282C1MG Innovative research each

Mouse Anti Monkeypox Virus M1R Monoclonal Clone SAA0283

IMSAMPXVM1RSAA0283C100UG Innovative research each

Mouse Anti Monkeypox Virus M1R Monoclonal Clone SAA0283

IMSAMPXVM1RSAA0283C1MG Innovative research each

Mouse Anti Monkeypox Virus M1R Monoclonal Clone SAA0284

IMSAMPXVM1RSAA0284C100UG Innovative research each

Mouse Anti Monkeypox Virus M1R Monoclonal Clone SAA0284

IMSAMPXVM1RSAA0284C1MG Innovative research each

Monkeypox Virus A35R Recombinant Protein N-GST and C-His Tag Lyophilized

IMPXVA35RRNGSTCHISLY100UG Innovative research each

Monkeypox Virus A35R Recombinant Protein N-GST and C-His Tag Lyophilized

IMPXVA35RRNGSTCHISLY1MG Innovative research each

Recombivirus Rabbit Reovirus sigma-3 (Reo-3/REOV) IgG ELISA Kit, 96 tests

AE-310720-1 Alpha Diagnostics 1 kit

Mouse Anti Monkeypox Virus A29L Monoclonal Clone SAA0286

IMSAMPXVA29LSAA0286C100UG Innovative research each

Mouse Anti Monkeypox Virus A29L Monoclonal Clone SAA0286

IMSAMPXVA29LSAA0286C1MG Innovative research each

Mouse Anti Monkeypox Virus A35R Monoclonal Clone SAA0287

IMSAMPXVA35RSAA0287C100UG Innovative research each

Mouse Anti Monkeypox Virus A35R Monoclonal Clone SAA0287

IMSAMPXVA35RSAA0287C1MG Innovative research each

RecombiVirus Mouse Anti-Rift Valley Fever Virus (RVFV) G2/Gc IgG ELISA kit, Quantitative, 96 tests

RV-404320-1 Alpha Diagnostics 1 kit

RecombiVirus Human Anti-Rift Valley Fever Virus (RVFV) G2/Gc IgG ELISA kit, Quantitative, 96 tests

RV-404330-1 Alpha Diagnostics 1 kit

RecombiVirus Mouse Polyomavirus (KV, Pneumotropic virus) IgG ELISA Kit, 96 tests

AE-300100-1 Alpha Diagnostics 1 kit

RecombiVirus Rabbit EDIM/rotavirus VP6 antibody IgG ELISA Kit, 96 tests, Quantitative

AE-300420-1 Alpha Diagnostics 1 kit

Recombinant Monkeypox virus Cu-Zn superoxide dismutase-like protein (A46R)

CSB-EP844975MHV Cusabio 20μg

Recombivirus? Human Anti-Zika Virus (ZIKV) Envelop domain III IgG ELISA kit, 96 tests, Quantitative

RV-403150-1 Alpha Diagnostics 1 Kit

Recombivirus? Human Anti-Zika Virus (ZIKV) Envelop domain III IgG ELISA kit, 96 tests, Quantitative

RV-403150-HG1 Alpha Diagnostics 1 Kit

Recombivirus? Human Anti-Zika Virus (ZIKV) Envelop domain III IgG ELISA kit, 96 tests, Quantitative

RV-403150-HG2 Alpha Diagnostics 1 Kit

Recombivirus? Mouse Anti-Zika Virus (ZIKV) Envelop domain III IgG ELISA kit, 96 tests, Quantitative

RV-403170-1 Alpha Diagnostics 1 Kit

Recombivirus? Rabbit Anti-Zika Virus (ZIKV) Envelop Domain III IgG ELISA kit, 96 tests, Quantitative

RV-403180-1 Alpha Diagnostics 1 Kit

Monkeypox Virus A29L (MPXV A29L) Antibody

abx376566-96tests Abbexa 96 tests

Monkeypox Virus A29L (MPXV A29L) Antibody

abx345842-100l Abbexa 100 µl

Monkeypox Virus A29L (MPXV A29L) Antibody

abx345842-50l Abbexa 50 µl

RecombiVirus Mouse Anti-Rift Valley Fever Virus (RVFV) NP IgG ELISA kit (DIVA), Quantitative, 96 tests

RV-404370-1 Alpha Diagnostics 1 kit

RecombiVirus Human Anti-Rift Valley Fever Virus (RVFV) NP IgG ELISA kit (DIVA), Quantitative, 96 tests

RV-404380-1 Alpha Diagnostics 1 kit

Recombivirus Rat Ectromelia virus (ECTV/mousepox/variola) IgG ELISA kit, 96 tests

AE-311110-1 Alpha Diagnostics 1 kit

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 Creative Biogene 1 unit

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 Gentaur Genprice 25 tests/kit

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 Gentaur Genprice 25 tests/kit

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T Jiangsu Bioperfectus Technologies 25 tests/kit

Monkeypox Virus (MPXV) RT-PCR Kit

abx471001-1096tests Abbexa 10 × 96 tests

Monkeypox Virus (MPXV) RT-PCR Kit

abx471001-596tests Abbexa 5 × 96 tests

Monkeypox Virus (MPXV) RT-PCR Kit

abx471001-96tests Abbexa 96 tests

Monkeypox Virus (MPXV) E8L ELISA Kit

RK04609 Abclonal 96T

RecombiVirus Mouse Lymphocytic choriomeningitis virus (LCMV) IgG ELISA Kit, 96 tests

AE-300200-1 Alpha Diagnostics 1 kit

Recombivirus Mouse Ectromelia virus (ECTV/mousepox/variola) IgG ELISA kit, 96 tests

AE-311100-1 Alpha Diagnostics 1 kit

Monkeypox virus (MPXV) E8L Rabbit mAb

A23087 Abclonal 500μL

Recombivirus? Camel Anti-Peste des petits ruminants NP IgG (PPR-NP) ELISA kit, Quantitative, 1x96 tests

RV-400820-1 Alpha Diagnostics 1 kit

Recombivirus? Camel Anti-Peste des petits ruminants NP IgG (PPR-NP) ELISA kit, Quantitative, 5x96 tests

RV-400820-5 Alpha Diagnostics 1 kit

Monkeypox Virus E8L (MPXV_USA_2022_MA001), mFc Tag

E24PVA019 EnoGene 50 μg

Monkeypox Virus P4a (MPXV P4a) Protein

abx160093-100g Abbexa 100 µg

Monkeypox Virus P4a (MPXV P4a) Protein

abx160093-10g Abbexa 10 µg

Monkeypox Virus P4a (MPXV P4a) Protein

abx160093-50g Abbexa 50 µg

Monkeypox Virus B5R (MPXV B5R) Protein

abx620096-100g Abbexa 100 µg

Monkeypox Virus B5R (MPXV B5R) Protein

abx620096-1mg Abbexa 1 mg

Monkeypox Virus B5R (MPXV B5R) Protein

abx620096-200g Abbexa 200 µg

Monkeypox Virus H3L (MPXV H3L) Protein

abx620102-100g Abbexa 100 µg
In this study, we inoculated CAST/EiJ mice with a range of VARV doses (102-106 plaque forming units). Some animals had detectable viable VARV from the oropharynx between days 3 and 12 post inoculation. Despite evidence of disease, the CAST/EiJ mouse does not provide a model for clinical smallpox due to mild signs of morbidity and limited skin lesions.
However, in contrast to previous rodent models using VARV challenge (i.e. prairie dogs and SCID mice), a robust immune response was observed in the CAST/EiJ mice (measured by Immunoglobulin G enzyme-linked immunosorbent assay). This is an advantage of this model for the study of VARV and presents a unique potential for the study of the immunomodulatory pathways following VARV infection.

Leave a comment