5-HIAA (5-Hydroxyindoleacetic Acid) Antibody

The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique.

The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose-dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTTP.

The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Photo Description: IHC image of neurons staining for 5-H1AA in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning.

Floating sections were incubated at RT in 10% goat serum in PBS, before the standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

  • Host: Rabbit
  • Quantity/Volume: 100 µL
  • State: Lyophilized Whole Serum
  • Reacts With: : Rat, Tritonia Diomedea (Sea Slug)
  • Alternate Names: anti-5-HIAA
  • RRID: AB_572208
  • Immunogen: 5-HIAA
  • Gene Symbol: SLC6A4
  • Entrez Gene ID: 6532
  • NCBI Gene Aliases: 5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSE

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